#Cellprofiler intensity measurements how to

The MMP assay is sensitive to FCCP even at the concentration of 0.01 µM. We could quantitatively analyze fluorescent images with high-throughput and high-speed detection to track the alteration of mitochondrial status. The CellProfiler image analysis software was further used to identify cell and mitochondrial phenotypes in the thousand fluorescent images. We employed the Operetta CLS TM to take fluorescent images with its given quickness and high resolution. The cells were co-stained with fluorescent dyes (Hoechst 33342 and MITO-ID® Red/MitoPY1/JC-10) and continuously processed by the High Content Imaging System.

#Cellprofiler intensity measurements how to

This pipeline demonstrates how to accurately identify these cells and how to measurements cellular parameters such as morphology, count, intensity and texture.

Human cells: Human HT29 cells are fairly smooth and elliptical. CellProfiler can address a variety of biological questions quantitatively, including standard assays (for example, cell count, size, per-cell protein levels) and complex morphological assays (for example, cell/organelle. Herein, we demonstrated an approach for determining the status of mitochondrial health by observing mitochondrial H 2O 2 (one type of ROS), membrane potential, and morphology (fragmentation and length) in live primary fibroblast cells. These pipelines are made for simple cellular and tissue image assays, and include some basic measurements. Here we describe the first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler. The investigation of mitochondrial heath is critical to evaluate the cellular status. Mitochondrial dysfunction relates to various pathological diseases.

Mitochondria are important organelles responsible for energy production.